H2AFY antibody (AA 36-85)

Catalog No. ABIN6741438

The Rabbit Polyclonal anti-H2AFY antibody has been validated for WB. It is suitable to detect H2AFY in samples from Human, Rat, Mouse, Cow, Horse, Dog, Guinea Pig, Rabbit, Pig, Monkey, Zebrafish (Danio rerio), Bat, Chicken and Xenopus laevis.

Quick Overview for H2AFY antibody (AA 36-85) (ABIN6741438)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Guinea Pig, Rabbit, Pig, Monkey, Zebrafish (Danio rerio), Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-H2AFY Antibody

Binding Specificity: AA 36-85

Specificity: Human H2AFY

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Chicken, Zebrafish (100%) Rabbit, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human H2AFY (O75367, NP_001035248). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Stickleback, Zebrafish (100%), Frog, Pike, Sea squirt, Drosophila, Grape, Arabidopsis (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: H2AFY / MACROH2A1

Background: Name/Gene ID: H2AFY

Synonyms: H2AFY, Core histone macro-H2A.1, Histone macroH2A1.1, H2A histone family, member Y, H2A.y, Histone H2A.y, MACROH2A1.1, MacroH2A1.2, H2AF12M, Histone macroH2A1.2, MACROH2A1, Histone macroH2A1, MH2A1

Gene ID: 9555

NCBI Accession: NP_001035248

UniProt: O75367