ACTN1 antibody (N-Term)

Catalog No. ABIN6741445

This Rabbit Polyclonal antibody specifically detects ACTN1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Monkey, Cow, Rabbit, Chicken, Dog, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for ACTN1 antibody (N-Term) (ABIN6741445)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Rabbit, Chicken, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACTN1 Antibody

Binding Specificity: N-Term

Specificity: Human ACTN1 / ASMA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken (100%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human ACTN1 (P12814, NP_001093). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Opossum, Guinea pig, Chicken (100%), Zebrafish (84%), Xenopus (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background: Name/Gene ID: ACTN1

Synonyms: ACTN1, Alpha-actinin-1, Actinin 1 smooth muscle, Actinin, alpha 1, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ASMA

Gene ID: 87

NCBI Accession: NP_001093

UniProt: P12814

Pathways: Cell-Cell Junction Organization