Calreticulin 3 antibody (N-Term)

Catalog No. ABIN6741470

The Rabbit Polyclonal anti-Calreticulin 3 antibody has been validated for WB. It is suitable to detect Calreticulin 3 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit, Monkey and Bat.

Quick Overview for Calreticulin 3 antibody (N-Term) (ABIN6741470)

Target: Calreticulin 3 (CALR3)

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin 3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Calreticulin 3 Antibody

Binding Specificity: N-Term

Specificity: Human CALR3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Mouse, Rat, Bovine (92%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human CALR3 (Q96L12, NP_659483). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Hamster, Bovine (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Calreticulin 3

Target: Calreticulin 3 (CALR3)

Alternative Name: CALR3

Background: Name/Gene ID: CALR3

Synonyms: CALR3, Calreticulin 3, Calreticulin-2, Calreticulin-3, Calsperin, CRT2, CT93, CMH19, Cancer/testis antigen 93

Gene ID: 125972

NCBI Accession: NP_659483

UniProt: Q96L12

Pathways: Activation of Innate immune Response