EN1 antibody (AA 252-301)

Catalog No. ABIN6741515

The Rabbit Polyclonal anti-EN1 antibody has been validated for WB. It is suitable to detect EN1 in samples from Human, Mouse, Chicken, Rat, Guinea Pig, Cow, Monkey and Pig.

Quick Overview for EN1 antibody (AA 252-301) (ABIN6741515)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Human, Mouse, Chicken, Rat, Guinea Pig, Cow, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EN1 Antibody

Binding Specificity: AA 252-301

Specificity: Human EN1

Predicted Reactivity: Percent identity by BLAST analysis: Pig, Guinea pig (93%) Xenopus (92%) Rabbit (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human EN1 (Q05925, NP_001417). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Bovine, Pig, Guinea pig, Chicken, Platypus, Lizard (100%), Gibbon, Galago, Elephant, Bat, Horse, Opossum (93%), Rabbit, Xenopus, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: EN1 / Engrailed

Background: Name/Gene ID: EN1

Synonyms: EN1, Engrailed 1, Engrailed homolog 1, Homeobox protein en-1, Homeobox protein engrailed-1, Hu-En-1, Engrailed homeobox 1

Gene ID: 2019

NCBI Accession: NP_001417

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis