EN2 antibody (C-Term)

Catalog No. ABIN6741528

The Rabbit Polyclonal anti-EN2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect EN2 in samples from Human, Mouse, Rat, Guinea Pig, Horse, Monkey and Pig.

Quick Overview for EN2 antibody (C-Term) (ABIN6741528)

Target: EN2 (Engrailed Homeobox 2 (EN2))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EN2 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human EN2

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Horse, Guinea pig (100%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EN2 (P19622, NP_001418). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Horse, Pig, Guinea pig (100%), Bat, Opossum, Zebra finch, Chicken, Platypus (92%), Xenopus, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.05 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EN2

Target: EN2 (Engrailed Homeobox 2 (EN2))

Alternative Name: EN2

Background: Name/Gene ID: EN2

Synonyms: EN2, AUTS10, Engrailed homolog 2, Engrailed homeobox 2, Homeobox protein engrailed-2, Engrailed-2, Homeobox protein en-2, Hu-En-2

Gene ID: 2020

NCBI Accession: NP_001418

UniProt: P19622