Vitamin D-Binding Protein antibody (AA 164-213)

Catalog No. ABIN6741530

This Rabbit Polyclonal antibody specifically detects Vitamin D-Binding Protein in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Horse, Dog, Pig, Cow, Monkey and Sheep.

Quick Overview for Vitamin D-Binding Protein antibody (AA 164-213) (ABIN6741530)

Target: Vitamin D-Binding Protein (GC)

Reactivity: Human, Mouse, Rat, Horse, Dog, Pig, Cow, Monkey, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vitamin D-Binding Protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Vitamin D-Binding Protein Antibody

Binding Specificity: AA 164-213

Brand: IHC-plus™

Specificity: Human DBP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa164-213 of human DBP (Q10586, NP_001343). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Dog, Bovine, Horse, Pig (100%), Elephant (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Vitamin D-Binding Protein

Target: Vitamin D-Binding Protein (GC)

Alternative Name: D-Binding Protein / DBP

Background: Name/Gene ID: DBP

Synonyms: DBP, D-binding protein, DABP, Albumin D box-binding protein, TaxREB302, D site-binding protein

Gene ID: 1628

NCBI Accession: NP_001343

UniProt: Q10586

Pathways: Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process