EMX2 antibody (N-Term)

Catalog No. ABIN6741532

This anti-EMX2 antibody is a Rabbit Polyclonal antibody detecting EMX2 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Bat, Monkey, Pig and Chicken.

Quick Overview for EMX2 antibody (N-Term) (ABIN6741532)

Target: EMX2 (Empty Spiracles Homeobox 2 (EMX2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Bat, Monkey, Pig, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EMX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EMX2 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human EMX2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig, Chicken (100%) Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human EMX2 (Q04743, NP_004089). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus (100%), Xenopus, Salmon, Medaka, Zebrafish (92%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EMX2

Target: EMX2 (Empty Spiracles Homeobox 2 (EMX2))

Alternative Name: EMX2

Background: Name/Gene ID: EMX2

Synonyms: EMX2, Empty spiracles homeobox 2, Empty spiracles-like protein 2, Homeobox protein EMX2, Empty spiracles homolog 2

Gene ID: 2018

NCBI Accession: NP_004089

UniProt: Q04743