GATA3 antibody (AA 36-85)

Catalog No. ABIN462169

This Rabbit Polyclonal antibody specifically detects GATA3 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig, Zebrafish (Danio rerio), Dog, Rabbit, Chicken, Horse, Sheep, Monkey and Hamster.

Quick Overview for GATA3 antibody (AA 36-85) (ABIN462169)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reactivity: Human, Mouse, Rat, Cow, Pig, Zebrafish (Danio rerio), Dog, Rabbit, Chicken, Horse, Sheep, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GATA3 Antibody

Binding Specificity: AA 36-85

Specificity: Human GATA3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Guinea pig (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human GATA3 (P23771, NP_002042). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%), Bat, Guinea pig, Trout, Salmon (92%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA3

Background: Name/Gene ID: GATA3
Family: Transcription factor

Synonyms: GATA3, GATA-binding factor 3, HDRS, GATA binding protein 3, GATA-binding protein 3, HDR

Gene ID: 2625

NCBI Accession: NP_002042

UniProt: P23771

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation