NAB1 antibody (AA 71-120)

Catalog No. ABIN6741537

The Rabbit Polyclonal anti-NAB1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NAB1 in samples from Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Chicken and Xenopus laevis.

Quick Overview for NAB1 antibody (AA 71-120) (ABIN6741537)

Target: NAB1 (NGFI-A Binding Protein 1 (EGR1 Binding Protein 1) (NAB1))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NAB1 Antibody

Binding Specificity: AA 71-120

Brand: IHC-plus™

Specificity: Human NAB1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus (100%) Dog (91%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human NAB1 (Q13506, NP_005957). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus (100%), Elephant (92%), Dog (91%), Stickleback, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NAB1

Target: NAB1 (NGFI-A Binding Protein 1 (EGR1 Binding Protein 1) (NAB1))

Alternative Name: NAB1

Background: Name/Gene ID: NAB1

Synonyms: NAB1, EGR1 binding protein 1, EGR-1-binding protein 1, NGFI-A-binding protein 1

Gene ID: 4664

NCBI Accession: NP_005957

UniProt: Q13506