Ceramide Synthase 2 antibody (AA 72-121)

Catalog No. ABIN6741543

This Rabbit Polyclonal antibody specifically detects Ceramide Synthase 2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Guinea Pig, Pig and Hamster.

Quick Overview for Ceramide Synthase 2 antibody (AA 72-121) (ABIN6741543)

Target: Ceramide Synthase 2 (CERS2)

Reactivity: Human, Guinea Pig, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ceramide Synthase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Ceramide Synthase 2 Antibody

Binding Specificity: AA 72-121

Brand: IHC-plus™

Specificity: Human LASS2

Predicted Reactivity: Percent identity by BLAST analysis: Pig (100%) Mouse, Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human CERS2 (Q96G23, NP_071358). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Hamster, Pig, Guinea pig (100%), Orangutan, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Rabbit, Horse, Opossum (92%), Bovine, Bat (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Ceramide Synthase 2

Target: Ceramide Synthase 2 (CERS2)

Alternative Name: L3 / LASS2

Background: Name/Gene ID: CERS2

Synonyms: CERS2, Ceramide synthase 2, LAG1 longevity assurance 2, L3, LASS2, SP260, TMSG1

Gene ID: 29956

NCBI Accession: NP_071358

UniProt: Q96G23