TCF21 antibody (AA 42-91)

Catalog No. ABIN462190

This Rabbit Polyclonal antibody specifically detects TCF21 in WB, IHC (p) and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Bat, Hamster and Pig.

Quick Overview for TCF21 antibody (AA 42-91) (ABIN462190)

Target: TCF21 (Transcription Factor 21 (TCF21))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Bat, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-TCF21 Antibody

Binding Specificity: AA 42-91

Brand: IHC-plus™

Specificity: Human TCF21

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa42-91 of human TCF21 (O43680, NP_003197). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Gibbon, Marmoset (92%), Opossum, Turkey, Zebra finch, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TCF21

Target: TCF21 (Transcription Factor 21 (TCF21))

Alternative Name: TCF21 / Epicardin

Background: Name/Gene ID: TCF21
Family: Transcription factor

Synonyms: TCF21, Capsulin, BHLHa23, Epicardin, Pod-1, Transcription factor 21, TCF-21, POD1, Podocyte-expressed 1

Gene ID: 6943

NCBI Accession: NP_003197

UniProt: O43680

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling