ATF4 antibody (N-Term)

Catalog No. ABIN462203

The Rabbit Polyclonal anti-ATF4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ATF4 in samples from Human and Mouse.

Quick Overview for ATF4 antibody (N-Term) (ABIN462203)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATF4 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Mouse ATF4

Predicted Reactivity: Percent identity by BLAST analysis: Mouse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of mouse ATF4 (Q61328, NP_033846). Percent identity by BLAST analysis: Mouse (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background: Name/Gene ID: ATF4
Family: Transcription factor

Synonyms: ATF4, CREB-2, CREB2, TXREB, DNA-binding protein TAXREB67, TAXREB67

Gene ID: 468

NCBI Accession: NP_033846

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response