SOX17 antibody (C-Term)

Catalog No. ABIN6741583

The Rabbit Polyclonal anti-SOX17 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SOX17 in samples from Human, Mouse, Rat and Dog.

Quick Overview for SOX17 antibody (C-Term) (ABIN6741583)

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SOX17 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human SOX17

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Guinea pig (92%) Mouse, Rat, Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SOX17 (Q9H6I2, NP_071899). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Elephant, Panda, Bat, Pig, Guinea pig (92%), Galago, Marmoset, Mouse, Rat, Dog (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SOX17

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Alternative Name: SOX17

Background: Name/Gene ID: SOX17
Family: Transcription factor

Synonyms: SOX17, Transcription factor SOX-17, VUR3

Gene ID: 64321

NCBI Accession: NP_071899

UniProt: Q9H6I2