JAZF1 antibody (C-Term)

Catalog No. ABIN6741585

This Rabbit Polyclonal antibody specifically detects JAZF1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Zebrafish (Danio rerio), Rat, Guinea Pig, Horse, Rabbit, Dog, Chicken, Cow, Monkey, Bat, Pig and Xenopus laevis.

Quick Overview for JAZF1 antibody (C-Term) (ABIN6741585)

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Reactivity: Human, Mouse, Zebrafish (Danio rerio), Rat, Guinea Pig, Horse, Rabbit, Dog, Chicken, Cow, Monkey, Bat, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAZF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JAZF1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human JAZF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human JAZF1 (Q86VZ6, NP_778231). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 7.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for JAZF1

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Alternative Name: JAZF1

Background: Name/Gene ID: JAZF1

Synonyms: JAZF1, ZNF802, TIP27, Zinc finger protein 802, JAZF zinc finger 1, TAK1-interacting protein 27

Gene ID: 221895

NCBI Accession: NP_778231

UniProt: Q86VZ6