RAE1 antibody (C-Term)

Catalog No. ABIN6741587

This anti-RAE1 antibody is a Rabbit Polyclonal antibody detecting RAE1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Pig, Xenopus laevis, Bat, Chicken, Hamster and Monkey.

Quick Overview for RAE1 antibody (C-Term) (ABIN6741587)

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Pig, Xenopus laevis, Bat, Chicken, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAE1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human RAE1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken, Xenopus (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human RAE1 (P78406, NP_001015885). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Seabass, Salmon, Stickleback, Zebrafish (92%), Sea squirt, Beetle (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAE1

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Alternative Name: MRNP41 / RAE1

Background: Name/Gene ID: RAE1

Synonyms: RAE1, DJ481F12.3, DJ800J21.1, Mnrp41, MRNA export factor, MRNA-binding protein, 41-kD, Rae1 protein homolog, MIG14, Migration-inducing gene 14, MRNA export protein, MRNP41

Gene ID: 8480

NCBI Accession: NP_001015885

UniProt: P78406

Pathways: SARS-CoV-2 Protein Interactome