BPIFA1 antibody (AA 101-150)

Catalog No. ABIN462235

This Rabbit Polyclonal antibody specifically detects BPIFA1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Guinea Pig, Bat, Monkey and Pig.

Quick Overview for BPIFA1 antibody (AA 101-150) (ABIN462235)

Target: BPIFA1 (BPI Fold Containing Family A, Member 1 (BPIFA1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BPIFA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BPIFA1 Antibody

Binding Specificity: AA 101-150

Brand: IHC-plus™

Specificity: Human PLUNC

Predicted Reactivity: Percent identity by BLAST analysis: Pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa101-150 of human BPIFA1 (Q9NP55, NP_057667). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Pig, Guinea pig (100%), Panda, Rabbit (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BPIFA1

Target: BPIFA1 (BPI Fold Containing Family A, Member 1 (BPIFA1))

Alternative Name: BPIFA1 / LUNX / PLUNC

Background: Name/Gene ID: BPIFA1

Synonyms: BPIFA1, BA49G10.5, Lung-specific protein X, LUNX, Ligand-binding protein RYA3, SPLUNC1, SPURT, Von Ebner protein Hl, LPLUNC3, NASG, PLUNC, Protein Plunc

Gene ID: 51297

NCBI Accession: NP_057667

UniProt: Q9NP55