GTPBP10 antibody (AA 254-303)
GTPBP10
Reactivity: Human, Mouse, Rat, Rabbit, Horse, Pig, Hamster, Monkey
WB, IHC, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
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- Target See all GTPBP10 Antibodies
- GTPBP10 (GTP-Binding Protein 10 (GTPBP10))
- Binding Specificity
- AA 254-303
- Reactivity
- Human, Mouse, Rat, Rabbit, Horse, Pig, Hamster, Monkey
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human GTPBP10
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig (100%) Dog, Guinea pig (92%) Chicken (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa254-303 of human GTPBP10 (A4D1E9, NP_149098). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Rabbit, Horse, Pig, Opossum, Armadillo, Lizard, Stickleback (100%), Dog, Bovine, Bat, Guinea pig (92%), Salmon (90%), Turkey, Zebra finch, Chicken, Platypus (85%), Zebrafish (84%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled Water.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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