SLC25A28 antibody (AA 290-339)

Catalog No. ABIN6741603

The Rabbit Polyclonal anti-SLC25A28 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SLC25A28 in samples from Human, Rat, Cow and Monkey.

Quick Overview for SLC25A28 antibody (AA 290-339) (ABIN6741603)

Target: SLC25A28 (Solute Carrier Family 25, Member 28 (SLC25A28))

Reactivity: Human, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC25A28 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SLC25A28 Antibody

Binding Specificity: AA 290-339

Brand: IHC-plus™

Specificity: Human SLC25A28

Predicted Reactivity: Percent identity by BLAST analysis: Dog, Rabbit, Pig, Guinea pig (92%) Rat, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa290-339 of human SLC25A28 (Q96A46, NP_112489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Mouse, Elephant, Panda, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (92%), Rat, Bovine, Bat, Opossum, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SLC25A28

Target: SLC25A28 (Solute Carrier Family 25, Member 28 (SLC25A28))

Alternative Name: SLC25A28

Background: Name/Gene ID: SLC25A28

Synonyms: SLC25A28, Mitoferrin 2, MRS4L, HMRS3/4, MFRN2, MRS3/4, Mitoferrin-2, NPD016

Gene ID: 81894

NCBI Accession: NP_112489

UniProt: Q96A46

Pathways: Transition Metal Ion Homeostasis