PODXL antibody (AA 301-350)

Catalog No. ABIN6741605

This Rabbit Polyclonal antibody specifically detects PODXL in WB, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for PODXL antibody (AA 301-350) (ABIN6741605)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PODXL Antibody

Binding Specificity: AA 301-350

Brand: IHC-plus™

Specificity: Human PODXL

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa301-350 of human PODXL (A6NHX8, NP_001018121). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey (100%), Gorilla (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: PODXL / Podocalyxin

Background: Name/Gene ID: PODXL

Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81

Gene ID: 5420

NCBI Accession: NP_001018121

UniProt: O00592

Pathways: Tube Formation