BMPER antibody (AA 501-550)

Catalog No. ABIN6741613

This anti-BMPER antibody is a Rabbit Polyclonal antibody detecting BMPER in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Bat and Chicken.

Quick Overview for BMPER antibody (AA 501-550) (ABIN6741613)

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Bat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMPER antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BMPER Antibody

Binding Specificity: AA 501-550

Brand: IHC-plus™

Specificity: Human BMPER

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Chicken (100%) Bovine (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa501-550 of human BMPER (Q8N8U9, NP_597725). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%), Marmoset, Bovine, Guinea pig (92%), Xenopus, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BMPER

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Alternative Name: BMPER

Background: Name/Gene ID: BMPER

Synonyms: BMPER, CRIM3, Crossveinless 2, CV-2, Crossveinless-2, HCV2, Protein crossveinless-2, CV2, KIAA1965

Gene ID: 168667

NCBI Accession: NP_597725

UniProt: Q8N8U9