METTL3 antibody (AA 503-552)

Catalog No. ABIN6741673

The Rabbit Polyclonal anti-METTL3 antibody has been validated for WB. It is suitable to detect METTL3 in samples from Human, Rat, Mouse, Dog, Rabbit, Horse, Guinea Pig, Cow, Zebrafish (Danio rerio), Pig, Bat, Hamster, Monkey and Xenopus laevis.

Quick Overview for METTL3 antibody (AA 503-552) (ABIN6741673)

Target: METTL3 (Methyltransferase Like 3 (METTL3))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Horse, Guinea Pig, Cow, Zebrafish (Danio rerio), Pig, Bat, Hamster, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-METTL3 Antibody

Binding Specificity: AA 503-552

Specificity: Human METTL3

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Zebrafish (100%) Goat, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa503-552 of human METTL3 (Q86U44, NP_062826). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus, Stickleback, Zebrafish (100%), Goat, Drosophila, Beetle (92%), Paramecium (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for METTL3

Target: METTL3 (Methyltransferase Like 3 (METTL3))

Alternative Name: METTL3 / M6A

Background: Name/Gene ID: METTL3

Synonyms: METTL3, M6A, Methyltransferase like 3, MTA70, Spo8, IME4, MRNA m(6)A methyltransferase, MT-A70

Gene ID: 56339

NCBI Accession: NP_062826

UniProt: Q86U44