Pleckstrin antibody (N-Term)

Catalog No. ABIN6741695

The Rabbit Polyclonal anti-Pleckstrin antibody has been validated for WB. It is suitable to detect Pleckstrin in samples from Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio), Bat and Pig.

Quick Overview for Pleckstrin antibody (N-Term) (ABIN6741695)

Target: Pleckstrin (PLEK)

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio), Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pleckstrin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Pleckstrin Antibody

Binding Specificity: N-Term

Specificity: Human PLEK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig, Zebrafish (100%) Dog, Horse, Chicken (92%) Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PLEK (P08567, NP_002655). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Bovine, Bat, Pig, Guinea pig, Lizard, Zebrafish (100%), Monkey, Elephant, Dog, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus, Trout, Salmon, Stickleback (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Pleckstrin

Target: Pleckstrin (PLEK)

Alternative Name: PLEK / Pleckstrin

Background: Name/Gene ID: PLEK

Synonyms: PLEK, Platelet 47 kDa protein, Pleckstrin, p47

Gene ID: 5341

NCBI Accession: NP_002655

UniProt: P08567

Pathways: Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process