Erythrocyte Ankyrin antibody (AA 863-912)

Catalog No. ABIN6741743

The Rabbit Polyclonal anti-Erythrocyte Ankyrin antibody has been validated for WB. It is suitable to detect Erythrocyte Ankyrin in samples from Human and Monkey.

Quick Overview for Erythrocyte Ankyrin antibody (AA 863-912) (ABIN6741743)

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Erythrocyte Ankyrin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Erythrocyte Ankyrin Antibody

Binding Specificity: AA 863-912

Specificity: Human ANK1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa863-912 of human ANK1 (P16157, NP_065210). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%), Galago, Mouse, Rat, Dog, Bat, Rabbit, Horse, Guinea pig (92%), Marmoset, Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Alternative Name: ANK1 / Ankyrin

Background: Name/Gene ID: ANK1

Synonyms: ANK1, ANK-1, Ankyrin-1, Ankyrin-R, Erythrocyte ankyrin, ANK, Ankyrin, Ankyrin 1, erythrocytic, SPH2, SPH1

Gene ID: 286

NCBI Accession: NP_065210

UniProt: P16157

Pathways: Synaptic Membrane