Glucagon antibody (AA 107-156)

Catalog No. ABIN6741779

This Rabbit Polyclonal antibody specifically detects Glucagon in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Monkey, Guinea Pig and Hamster.

Quick Overview for Glucagon antibody (AA 107-156) (ABIN6741779)

Target: Glucagon (GCG)

Reactivity: Human, Rat, Mouse, Dog, Monkey, Guinea Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucagon antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Glucagon Antibody

Binding Specificity: AA 107-156

Specificity: Human GCG / Glucagon

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Horse, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human GCG (P01275, NP_002045). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Opossum, Guinea pig (100%), Sheep, Elephant, Bovine, Horse, Pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Glucagon

Target: Glucagon (GCG)

Alternative Name: GCG / Glucagon

Background: Name/Gene ID: GCG
Family: Hormone

Synonyms: GCG, GLP2, Glucagon, Glucagon-like peptide 2, GRPP, Glicentin-related polypeptide, GLP1, Glucagon-like peptide 1

Gene ID: 2641

NCBI Accession: NP_002045

UniProt: P01275

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling