EIF1AX antibody (C-Term)

Catalog No. ABIN6741786

The Rabbit Polyclonal anti-EIF1AX antibody has been validated for WB. It is suitable to detect EIF1AX in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio), Chicken, Hamster, Monkey, Pig and Xenopus laevis.

Quick Overview for EIF1AX antibody (C-Term) (ABIN6741786)

Target: EIF1AX (Eukaryotic Translation Initiation Factor 1A, X-Linked (EIF1AX))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio), Chicken, Hamster, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF1AX antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF1AX Antibody

Binding Specificity: C-Term

Specificity: Human EIF1AX

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Sheep, Bovine, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EIF1AX (P47813, NP_001403). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Sablefish, Stickleback, Zebrafish (100%), Bat (92%), Trout, Salmon, Pike (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:2500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF1AX

Target: EIF1AX (Eukaryotic Translation Initiation Factor 1A, X-Linked (EIF1AX))

Alternative Name: EIF1A / EIF1AX

Background: Name/Gene ID: EIF1AX

Synonyms: EIF1AX, EIF-1A X isoform, EIF4C, EIF1A, EIF-1A, EIF-4C, EIF1AP1

Gene ID: 1964

NCBI Accession: NP_001403

UniProt: P47813