GLO1 antibody (AA 35-84)

Catalog No. ABIN6741835

This Rabbit Polyclonal antibody specifically detects GLO1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Saccharomyces cerevisiae, Guinea Pig, Zebrafish (Danio rerio), Chicken and Monkey.

Quick Overview for GLO1 antibody (AA 35-84) (ABIN6741835)

Target: GLO1 (Glyoxalase I (GLO1))

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Guinea Pig, Zebrafish (Danio rerio), Chicken, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLO1 Antibody

Binding Specificity: AA 35-84

Specificity: Human GLO1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Guinea pig, Chicken (100%) Dog, Bovine, Rabbit, Horse, Pig (92%) Xenopus, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human GLO1 (Q04760, NP_006699). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Elephant, Dog, Bovine, Rabbit, Horse, Pig (92%), Xenopus, Smelt, Sablefish, Zebrafish, Grape, Arabidopsis (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GLO1

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: GLO1 / Glyoxalase I

Background: Name/Gene ID: GLO1

Synonyms: GLO1, Aldoketomutase, Glx I, Glyoxalase domain containing 1, Glyoxalase I, Lactoyl glutathione lyase, Lactoylglutathione lyase, GLOD1, Methylglyoxalase, Ketone-aldehyde mutase, GLYI

Gene ID: 2739

NCBI Accession: NP_006699

UniProt: Q04760