Phospholipase C beta 1 antibody (C-Term)

Catalog No. ABIN6741840

The Rabbit Polyclonal anti-Phospholipase C beta 1 antibody has been validated for WB. It is suitable to detect Phospholipase C beta 1 in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Rabbit, Pig, Monkey and Xenopus laevis.

Quick Overview for Phospholipase C beta 1 antibody (C-Term) (ABIN6741840)

Target: Phospholipase C beta 1 (PLCB1) (phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Rabbit, Pig, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C beta 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phospholipase C beta 1 Antibody

Binding Specificity: C-Term

Specificity: Human PLCB1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human PLCB1 (D3DW13, NP_877398). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Horse (92%), Elephant (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Phospholipase C beta 1

Target: Phospholipase C beta 1 (PLCB1) (phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1))

Alternative Name: PLCB1 / Phospholipase C Beta 1

Background: Name/Gene ID: PLCB1
Family: Phospholipase

Synonyms: PLCB1, EIEE12, KIAA0581, Phospholipase C Beta 1, Phospholipase C-I, Phosphoinositidase C, PI-PLC, Inositoltrisphosphohydrolase, PLC-I, PLCB1A, PLC-beta-1, Phospholipase C-beta-1, PLC154, PLC-154, PLCB1B

Gene ID: 23236

NCBI Accession: NP_877398

UniProt: Q9NQ66

Pathways: WNT Signaling, AMPK Signaling, Thyroid Hormone Synthesis, Inositol Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Proton Transport, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, G-protein mediated Events