IARS antibody (AA 35-84)

Catalog No. ABIN6741923

This Rabbit Polyclonal antibody specifically detects IARS in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Pig and Zebrafish (Danio rerio).

Quick Overview for IARS antibody (AA 35-84) (ABIN6741923)

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IARS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IARS Antibody

Binding Specificity: AA 35-84

Specificity: Human IARS

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Bovine, Rabbit, Guinea pig (92%) Mouse, Rat, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human IARS (P41252, NP_038203). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Dog, Pig (100%), Galago, Bovine, Bat, Rabbit, Guinea pig (92%), Drosophila (91%), Mouse, Rat, Hamster, Elephant, Horse, Platypus (85%), Beetle (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for IARS

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Alternative Name: IRS

Background: Name/Gene ID: IARS

Synonyms: IARS, IARS1, ILRS, IRS, Isoleucyl-tRNA synthetase, PRO0785, ILERS

Gene ID: 3376

NCBI Accession: NP_038203

UniProt: P41252