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HHAT antibody (N-Term)

This Rabbit Polyclonal antibody specifically detects HHAT in WB. It exhibits reactivity toward Human, Mouse, Guinea Pig, Horse, Rabbit and Monkey.
Catalog No. ABIN6742033

Quick Overview for HHAT antibody (N-Term) (ABIN6742033)

Target

See all HHAT Antibodies
HHAT (Hedgehog Acyltransferase (HHAT))

Reactivity

  • 27
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Guinea Pig, Horse, Rabbit, Monkey

Host

  • 27
Rabbit

Clonality

  • 27
Polyclonal

Conjugate

  • 8
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This HHAT antibody is un-conjugated

Application

  • 27
  • 13
  • 13
  • 9
  • 8
  • 7
  • 5
  • 3
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 7
    • 4
    • 1
    • 1
    N-Term

    Specificity

    Human HHAT

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rabbit, Horse, Guinea pig (100%) Pig (92%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from N-Terminus of human HHAT (Q5VTY9, NP_060664). Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Mouse, Elephant, Rabbit, Horse, Guinea pig (100%), Galago, Pig (92%), Opossum (91%), Bat, Xenopus (84%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    HHAT (Hedgehog Acyltransferase (HHAT))

    Alternative Name

    Skn / HHAT

    Background

    Name/Gene ID: HHAT

    Synonyms: HHAT, Gup2, MART-2, MART2, Hedgehog acyltransferase, Rasp, Skinny hedgehog protein 1, SIT, SKI1, Skn

    Gene ID

    55733

    NCBI Accession

    NP_060664

    UniProt

    Q5VTY9

    Pathways

    Hedgehog Signaling
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