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OTUB1 antibody (AA 71-120)

This Rabbit Polyclonal antibody specifically detects OTUB1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig and Monkey.
Catalog No. ABIN6742051

Quick Overview for OTUB1 antibody (AA 71-120) (ABIN6742051)

Target

See all OTUB1 Antibodies
OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

Reactivity

  • 62
  • 32
  • 18
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 1
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Monkey

Host

  • 73
  • 7
  • 1
Rabbit

Clonality

  • 73
  • 8
Polyclonal

Conjugate

  • 39
  • 13
  • 10
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This OTUB1 antibody is un-conjugated

Application

  • 70
  • 30
  • 21
  • 18
  • 13
  • 13
  • 12
  • 10
  • 6
  • 4
  • 3
  • 3
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 13
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 71-120

    Specificity

    Human OTUB1

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig (100%) Zebrafish (92%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa71-120 of human OTUB1 (Q96FW1, NP_060140). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Pig, Opossum, Guinea pig, Catfish, Salmon, Stickleback, Pike (100%), Zebrafish (92%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

    Alternative Name

    OTUB1 / OTU1

    Background

    Name/Gene ID: OTUB1

    Synonyms: OTUB1, Deubiquitinating enzyme OTUB1, HSPC263, HOTU1, OTU-domain Ubal-binding 1, OTU1, Otubain-1, Ubiquitin thioesterase OTUB1, OTB1

    Gene ID

    55611

    NCBI Accession

    NP_060140

    UniProt

    Q96FW1
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