BCAT1 antibody (N-Term)

Catalog No. ABIN6742066

The Rabbit Polyclonal anti-BCAT1 antibody has been validated for WB. It is suitable to detect BCAT1 in samples from Human, Mouse, Dog, Rabbit, Monkey and Bat.

Quick Overview for BCAT1 antibody (N-Term) (ABIN6742066)

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Reactivity: Human, Mouse, Dog, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BCAT1 Antibody

Binding Specificity: N-Term

Specificity: Human BCAT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit (100%) Horse (92%) Sheep, Chicken (91%) Mouse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human BCAT1 (P54687, NP_005495). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Elephant, Dog, Bat, Rabbit, Opossum (100%), Galago, Horse (92%), Sheep, Turkey, Chicken, Platypus (91%), Mouse (84%), Lizard (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BCAT1

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Alternative Name: BCAT1 / ECA39

Background: Name/Gene ID: BCAT1

Synonyms: BCAT1, BCAT(c), BCATC, ECA39, MECA39, BCT1, Placental protein 18, PNAS121, PP18, Protein ECA39

Gene ID: 586

NCBI Accession: NP_005495

UniProt: P54687