A4GALT antibody (AA 215-264)

Catalog No. ABIN6742071

The Rabbit Polyclonal anti-A4GALT antibody has been validated for WB. It is suitable to detect A4GALT in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Monkey, Chicken and Xenopus laevis.

Quick Overview for A4GALT antibody (AA 215-264) (ABIN6742071)

Target: A4GALT (alpha 1, 4-Galactosyltransferase (A4GALT))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Monkey, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This A4GALT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-A4GALT Antibody

Binding Specificity: AA 215-264

Specificity: Human A4GALT

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human A4GALT (Q9NPC4, NP_059132). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Guinea pig, Turkey, Chicken, Platypus, Xenopus (100%), Galago, Zebra finch (92%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for A4GALT

Target: A4GALT (alpha 1, 4-Galactosyltransferase (A4GALT))

Alternative Name: A4GALT

Background: Name/Gene ID: A4GALT

Synonyms: A4GALT, A4GALT1, A14GALT, Alpha4Gal-T1, CD77 synthase, Globotriaosylceramide synthase, P(k) antigen synthase, p1/Pk synthase, P blood group (P one antigen), P(k), p1, Gb3 synthase, Gb3S, PK

Gene ID: 53947

NCBI Accession: NP_059132

UniProt: Q9NPC4