WIPI1 antibody (AA 359-408)

Catalog No. ABIN6742099

This Rabbit Polyclonal antibody specifically detects WIPI1 in WB. It exhibits reactivity toward Human, Mouse, Horse, Dog, Rabbit and Bat.

Quick Overview for WIPI1 antibody (AA 359-408) (ABIN6742099)

Target: WIPI1 (WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1))

Reactivity: Human, Mouse, Horse, Dog, Rabbit, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WIPI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WIPI1 Antibody

Binding Specificity: AA 359-408

Specificity: Human WIPI1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit (100%) Rat, Bovine, Pig, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa359-408 of human WIPI1 (Q5MNZ9, NP_060453). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Elephant, Dog, Bat, Rabbit, Horse (100%), Galago, Rat, Bovine, Pig, Opossum, Guinea pig (92%), Marmoset (91%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for WIPI1

Target: WIPI1 (WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1))

Alternative Name: ATG18 / WIPI1

Background: Name/Gene ID: WIPI1

Synonyms: WIPI1, ATG18, Atg18 protein homolog, ATG18A, WIPI49, WIPI-1, WIPI 49 kDa, WIPI-1 alpha

Gene ID: 55062

NCBI Accession: NP_060453

UniProt: Q5MNZ9

Pathways: Nuclear Hormone Receptor Binding, ER-Nucleus Signaling