VPS53 antibody (AA 431-480)

Catalog No. ABIN6742156

The Rabbit Polyclonal anti-VPS53 antibody is suitable to detect VPS53 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse, Rabbit and Hamster. It has been validated for WB.

Quick Overview for VPS53 antibody (AA 431-480) (ABIN6742156)

Target: VPS53 (Vacuolar Protein Sorting 53 Homolog (VPS53))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS53 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VPS53 Antibody

Binding Specificity: AA 431-480

Specificity: Human VPS53

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse (100%) Dog, Guinea pig, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa431-480 of human VPS53 (B3FH42, NP_001121631). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Mouse, Rat, Hamster, Panda, Bovine, Rabbit, Horse, Opossum, Platypus (100%), Elephant, Dog, Bat, Guinea pig, Zebra finch, Chicken (92%), Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for VPS53

Target: VPS53 (Vacuolar Protein Sorting 53 Homolog (VPS53))

Alternative Name: HCCS1 / VPS53

Background: Name/Gene ID: VPS53

Synonyms: VPS53, HCCS1, HVps53L, Pp13624

Gene ID: 55275

NCBI Accession: NP_001121631

UniProt: Q5VIR6