ESR2 antibody (AA 396-445)

Catalog No. ABIN6742259

The Rabbit Polyclonal anti-ESR2 antibody has been validated for WB. It is suitable to detect ESR2 in samples from Human, Mouse, Rat, Sheep, Pig, Monkey, Dog, Cow, Rabbit and Guinea Pig.

Quick Overview for ESR2 antibody (AA 396-445) (ABIN6742259)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human, Mouse, Rat, Sheep, Pig, Monkey, Dog, Cow, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ESR2 Antibody

Binding Specificity: AA 396-445

Specificity: Human ESR2 / ER-Beta

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human ESR2 (Q92731, NP_001428). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey (100%), Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: ESR2 / ER Beta

Background: Name/Gene ID: ESR2
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR2, ESTRB, ER Beta, ERbeta, ER-BETA, ESR-BETA, ESRB, Estrogen receptor 2 (ER beta), NR3A2, Estrogen receptor beta, Estrogen receptor beta 4

Gene ID: 2100

NCBI Accession: NP_001428

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling