SIAH2 antibody (AA 215-264)

Catalog No. ABIN6742365

The Rabbit Polyclonal anti-SIAH2 antibody has been validated for WB. It is suitable to detect SIAH2 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Pig, Guinea Pig, Horse, Rabbit, Monkey, Bat, Chicken and Xenopus laevis.

Quick Overview for SIAH2 antibody (AA 215-264) (ABIN6742365)

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Pig, Guinea Pig, Horse, Rabbit, Monkey, Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIAH2 Antibody

Binding Specificity: AA 215-264

Specificity: Human SIAH2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human SIAH2 (O43255, NP_005058). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Xenopus, Zebrafish (100%), Hamster, Panda, Zebra finch, Stickleback, Drosophila (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIAH2

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Alternative Name: SIAH2

Background: Name/Gene ID: SIAH2

Synonyms: SIAH2, HSiah2, Seven in absentia homolog 2, Siah-2

Gene ID: 6478

NCBI Accession: NP_005058

UniProt: O43255