RUNX1 antibody (C-Term)

Catalog No. ABIN6742744

The Rabbit Polyclonal anti-RUNX1 antibody has been validated for WB. It is suitable to detect RUNX1 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig, Monkey and Zebrafish (Danio rerio).

Quick Overview for RUNX1 antibody (C-Term) (ABIN6742744)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig, Monkey, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX1 Antibody

Binding Specificity: C-Term

Specificity: Human RUNX1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken, Xenopus (92%) Mouse, Rat, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human RUNX1 (B2RMS4, NP_001745). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Galago, Elephant, Opossum, Turkey, Zebra finch, Chicken, Xenopus (92%), Mouse, Rat, Platypus, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: AML1 / RUNX1

Background: Name/Gene ID: RUNX1

Synonyms: RUNX1, AMLCR1, Aml1 oncogene, AML1, AML1-EVI-1, CBF-alpha-2, CBFA2, Acute myeloid leukemia 1, EVI-1, PEBP2A2, PEA2-alpha B, AML1-EVI-1 fusion protein, Oncogene AML-1, PEBP2-alpha B, PEBP2aB

Gene ID: 861

NCBI Accession: NP_001745

UniProt: Q01196