OCT4 antibody (AA 33-82)

Catalog No. ABIN6742947

This anti-OCT4 antibody is a Rabbit Polyclonal antibody detecting OCT4 in WB. Suitable for Human, Rat, Pig, Cow, Dog, Rabbit, Monkey, Goat, Sheep and Hamster.

Quick Overview for OCT4 antibody (AA 33-82) (ABIN6742947)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Rat, Pig, Cow, Dog, Rabbit, Monkey, Goat, Sheep, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-OCT4 Antibody

Binding Specificity: AA 33-82

Specificity: Human POU5F1 / OCT4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Goat, Rabbit, Pig (100%) Mouse, Rat (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa33-82 of human POU5F1 (Q01860-2, NP_976034). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Sheep, Goat, Hamster, Dog, Cat, Bovine, Rabbit, Pig (100%), Mouse, Horse (92%), Platypus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1 / OCT4

Background: Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

Gene ID: 5460

NCBI Accession: NP_976034

UniProt: Q01860

Pathways: Stem Cell Maintenance