ube3a antibody (AA 180-229)

Catalog No. ABIN6743132

This anti-ube3a antibody is a Rabbit Polyclonal antibody detecting ube3a in WB. Suitable for Human, Rat, Zebrafish (Danio rerio), Monkey, Bat and Drosophila melanogaster.

Quick Overview for ube3a antibody (AA 180-229) (ABIN6743132)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human, Rat, Zebrafish (Danio rerio), Monkey, Bat, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ube3a Antibody

Binding Specificity: AA 180-229

Specificity: Human UBE3A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Zebrafish (100%) Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human UBE3A (Q05086, NP_000453). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Zebrafish, Drosophila (100%), Galago, Mouse, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: E6AP / UBE3A

Background: Name/Gene ID: UBE3A

Synonyms: UBE3A, ANCR, E6-ap isoform-III, E6AP ubiquitin-protein ligase, EPVE6AP, HPVE6A, AS, CTCL tumor antigen se37-2, E6-AP, E6AP, Ubiquitin protein ligase E3A, Ubiquitin-protein ligase E3A

Gene ID: 7337

NCBI Accession: NP_000453

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway