HNF4A antibody (AA 252-301)

Catalog No. ABIN6743376

The Rabbit Polyclonal anti-HNF4A antibody has been validated for WB. It is suitable to detect HNF4A in samples from Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Horse, Rabbit, Guinea Pig, Sheep, Bat and Hamster.

Quick Overview for HNF4A antibody (AA 252-301) (ABIN6743376)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Horse, Rabbit, Guinea Pig, Sheep, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HNF4A Antibody

Binding Specificity: AA 252-301

Specificity: Human HNF4A / HNF4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human HNF4A (P41235, NP_001025174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Stickleback (100%), Elephant, Turkey, Chicken, Platypus, Lizard, Xenopus, Catfish, Zebrafish (92%), Zebra finch (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A / HNF4

Background: Name/Gene ID: HNF4A
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: HNF4A, Hepatic nuclear factor 4 alpha, HNF4, HNF4a8, NR2A21, TCF, TCF-14, TCF14, MODY1, Transcription factor 14, Transcription factor HNF-4, HNF-4-alpha, HNF4 alpha, HNF4a7, HNF4a9, HNF4alpha, HNF4alpha10/11/12, MODY, NR2A1

Gene ID: 3172

NCBI Accession: NP_001025174

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process