RhoF antibody (AA 107-156)

Catalog No. ABIN6744166

The Rabbit Polyclonal anti-RhoF antibody has been validated for WB. It is suitable to detect RhoF in samples from Human, Mouse, Cow, Sheep, Pig, Horse, Dog, Guinea Pig, Rabbit, Monkey, Hamster, Chicken and Bat.

Quick Overview for RhoF antibody (AA 107-156) (ABIN6744166)

Target: RhoF (RHOF) (Rho-related GTP-binding protein RhoF (RHOF))

Reactivity: Human, Mouse, Cow, Sheep, Pig, Horse, Dog, Guinea Pig, Rabbit, Monkey, Hamster, Chicken, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RhoF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RhoF Antibody

Binding Specificity: AA 107-156

Specificity: Human RHOF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Sheep, Dog, Bovine, Rabbit, Pig, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human RHOF (Q9HBH0, NP_061907). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Sheep, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch, Chicken, Platypus (100%), Opossum (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RhoF

Target: RhoF (RHOF) (Rho-related GTP-binding protein RhoF (RHOF))

Alternative Name: RIF / RHOF

Background: Name/Gene ID: RHOF
Family: Ras GTPase superfamily IPR001806

Synonyms: RHOF, ARHF, Rho family GTPase Rif, Rho in filopodia, RIF

Gene ID: 54509

NCBI Accession: NP_061907

UniProt: Q9HBH0