FLIP antibody (AA 180-229)

Catalog No. ABIN6744339

The Rabbit Polyclonal anti-FLIP antibody has been validated for WB. It is suitable to detect FLIP in samples from Human and Monkey.

Quick Overview for FLIP antibody (AA 180-229) (ABIN6744339)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FLIP Antibody

Binding Specificity: AA 180-229

Specificity: Human CFLAR / FLIP

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human CFLAR (O15519, NP_003870). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR / FLIP

Background: Name/Gene ID: CFLAR
Subfamily: Cysteine C14
Family: Apoptosis

Synonyms: CFLAR, C-FLIPR, C-FLIPS, C-FLIP, CASP8AP1, Cflip, CLARP, CASH, Caspase homolog, Caspase-eight-related protein, Flice inhibitory protein, I-FLICE, Inhibitor of FLICE, FLAME, FLAME1, MRIT, Usurpin beta, C-FLIPL, Casper, FLAME-1, FLIP, Usurpin

Gene ID: 8837

NCBI Accession: NP_003870

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development