YWHAE antibody (C-Term)

Catalog No. ABIN6744347

The Rabbit Polyclonal anti-YWHAE antibody has been validated for WB. It is suitable to detect YWHAE in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Sheep, Guinea Pig, Chicken, Horse, Monkey, Rabbit, Dog and Bat.

Quick Overview for YWHAE antibody (C-Term) (ABIN6744347)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Sheep, Guinea Pig, Chicken, Horse, Monkey, Rabbit, Dog, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-YWHAE Antibody

Binding Specificity: C-Term

Specificity: Human YWHAE / 14-3-3 Epsilon

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Dog, Guinea pig, Chicken, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human YWHAE (P62258, NP_006752). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Salmon, Zebrafish (100%), Gibbon, Galago, Xenopus, Trout, Catfish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE / 14-3-3 Epsilon

Background: Name/Gene ID: YWHAE

Synonyms: YWHAE, 14-3-3E, 14-3-3 epsilon, 14-3-3 protein epsilon, MDCR, MDS, KCIP-1

Gene ID: 7531

NCBI Accession: NP_006752

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase