GRHL2 antibody (C-Term)

Catalog No. ABIN6744427

This anti-GRHL2 antibody is a Rabbit Polyclonal antibody detecting GRHL2 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Chicken, Bat, Monkey, Pig and Xenopus laevis.

Quick Overview for GRHL2 antibody (C-Term) (ABIN6744427)

Target: GRHL2 (Grainyhead-Like 2 (GRHL2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Chicken, Bat, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRHL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRHL2 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human GRHL2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human GRHL2 (Q6ISB3, NP_079191). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Zebrafish (100%), Stickleback (92%), Orangutan, Sparrow, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GRHL2

Target: GRHL2 (Grainyhead-Like 2 (GRHL2))

Alternative Name: GRHL2

Background: Name/Gene ID: GRHL2

Synonyms: GRHL2, DFNA28, BOM, Grainyhead-like 2 (Drosophila), TFCP2L3

Gene ID: 79977

NCBI Accession: NP_079191

UniProt: Q6ISB3

Pathways: Tube Formation