Desmoglein 2 antibody (AA 72-121)

Catalog No. ABIN6744433

The Rabbit Polyclonal anti-Desmoglein 2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Desmoglein 2 in samples from Human, Rat, Mouse and Monkey.

Quick Overview for Desmoglein 2 antibody (AA 72-121) (ABIN6744433)

Target: Desmoglein 2 (DSG2)

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Desmoglein 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Desmoglein 2 Antibody

Binding Specificity: AA 72-121

Brand: IHC-plus™

Specificity: Human DSG2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Rabbit, Horse, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human DSG2 (Q14126, NP_001934). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat (100%), Elephant, Bovine, Rabbit, Horse, Guinea pig (92%), Bat (85%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: DSG2 / Desmoglein 2

Background: Name/Gene ID: DSG2
Family: Cadherin

Synonyms: DSG2, ARVC10, Cadherin family member 5, CDHF5, CMD1BB, Desmoglein-2, Desmoglein II, HDGC, ARVD10, Desmoglein 2

Gene ID: 1829

NCBI Accession: NP_001934

UniProt: Q14126

Pathways: Cell-Cell Junction Organization