GPSM2 antibody (AA 72-121)

Catalog No. ABIN6744437

This Rabbit Polyclonal antibody specifically detects GPSM2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Monkey and Bat.

Quick Overview for GPSM2 antibody (AA 72-121) (ABIN6744437)

Target: GPSM2 (G-Protein Signaling Modulator 2 (GPSM2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPSM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPSM2 Antibody

Binding Specificity: AA 72-121

Brand: IHC-plus™

Specificity: Human GPSM2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Zebrafish (100%) Xenopus (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human GPSM2 (P81274, NP_037428). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Zebrafish (100%), Zebra finch, Platypus, Xenopus (92%), Hamster, Panda, Guinea pig, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IF

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GPSM2

Target: GPSM2 (G-Protein Signaling Modulator 2 (GPSM2))

Alternative Name: GPSM2 / LGN

Background: Name/Gene ID: GPSM2

Synonyms: GPSM2, DFNB82, LGN, PINS, CMCS, Mosaic protein LGN

Gene ID: 29899

NCBI Accession: NP_037428

UniProt: P81274

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling