MCTP1 antibody (C-Term)

Catalog No. ABIN6744450

This Rabbit Polyclonal antibody specifically detects MCTP1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Guinea Pig, Horse, Zebrafish (Danio rerio), Chicken, Monkey and Pig.

Quick Overview for MCTP1 antibody (C-Term) (ABIN6744450)

Target: MCTP1 (Multiple C2 Domains, Transmembrane 1 (MCTP1))

Reactivity: Human, Mouse, Guinea Pig, Horse, Zebrafish (Danio rerio), Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCTP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MCTP1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human MCTP1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Horse, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MCTP1 (Q6DN14-2, NP_001002796). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Horse, Pig, Opossum, Guinea pig, Chicken, Lizard (100%), Panda, Dog, Turkey (92%), Rat, Bat, Rabbit, Zebra finch, Platypus, Xenopus, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MCTP1

Target: MCTP1 (Multiple C2 Domains, Transmembrane 1 (MCTP1))

Alternative Name: MCTP1

Background: Name/Gene ID: MCTP1

Synonyms: MCTP1

Gene ID: 79772

NCBI Accession: NP_001002796

UniProt: Q6DN14