GABARAPL1 antibody (N-Term)

Catalog No. ABIN6744456

The Rabbit Polyclonal anti-GABARAPL1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect GABARAPL1 in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Pig, Chicken, Hamster, Monkey and Xenopus laevis.

Quick Overview for GABARAPL1 antibody (N-Term) (ABIN6744456)

Target: GABARAPL1 (GABA(A) Receptor-Associated Protein Like 1 (GABARAPL1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Pig, Chicken, Hamster, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAPL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GABARAPL1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human GABARAPL1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human GABARAPL1 (Q9H0R8, NP_113600). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GABARAPL1

Target: GABARAPL1 (GABA(A) Receptor-Associated Protein Like 1 (GABARAPL1))

Alternative Name: GABARAPL1 / ATG8

Background: Name/Gene ID: GABARAPL1

Synonyms: GABARAPL1, ATG8, ATG8L, APG8L, GABARAPL1-a, GEC-1, GEC1, APG8-LIKE, ATG8B

Gene ID: 23710

NCBI Accession: NP_113600

UniProt: Q9H0R8

Pathways: Autophagy