FOXM1 antibody (C-Term)

Catalog No. ABIN490076

The Rabbit Polyclonal anti-FOXM1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FOXM1 in samples from Human, Dog, Guinea Pig and Pig.

Quick Overview for FOXM1 antibody (C-Term) (ABIN490076)

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Reactivity: Human, Dog, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXM1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human FOXM1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Rabbit (91%) Dog, Pig, Guinea pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human FOXM1 (Q08050, NP_068772). Percent identity by BLAST analysis: Human, Chimpanzee (100%), Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Rabbit (91%), Elephant (90%), Galago, Panda, Dog, Pig, Guinea pig (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXM1

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Alternative Name: FOXM1

Background: Name/Gene ID: FOXM1
Family: Transcription factor

Synonyms: FOXM1, Forkhead box M1, Forkhead box protein M1, HFH11, HNF-3, HNF-3/fork-head homolog 11, INS-1, FOXM1B, M-phase phosphoprotein 2, MPP-2, MPHOSPH2, PIG29, TRIDENT, FKHL16, HFH-11, TGT3, Transcription factor Trident, WIN

Gene ID: 2305

NCBI Accession: NP_068772

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus