KIF20A antibody (C-Term)

Catalog No. ABIN6744463

This anti-KIF20A antibody is a Rabbit Polyclonal antibody detecting KIF20A in WB, IHC and IHC (p). Suitable for Human, Rat, Dog, Horse, Pig, Rabbit and Cow.

Quick Overview for KIF20A antibody (C-Term) (ABIN6744463)

Target: KIF20A (Kinesin Family Member 20A (KIF20A))

Reactivity: Human, Rat, Dog, Horse, Pig, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF20A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KIF20A Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human KIF20A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig (100%) Chicken (92%) Rat (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human KIF20A (O95235, NP_005724). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%), Galago, Marmoset, Mouse, Bat, Guinea pig (93%), Turkey, Chicken (92%), Rat (86%), Zebra finch (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KIF20A

Target: KIF20A (Kinesin Family Member 20A (KIF20A))

Alternative Name: RAB6KIFL / KIF20A

Background: Name/Gene ID: KIF20A

Synonyms: KIF20A, Kinesin family member 20A, Kinesin-like protein KIF20A, GG10_2, Mitotic kinesin-like protein 2, MKLP2, Rabkinesin-6, RAB6KIFL

Gene ID: 10112

NCBI Accession: NP_005724

UniProt: O95235